Content Loading...
Content Loading...
Product Reference
Rapid isolation and detection of microbial contaminants in manufacturing
| |
|
| Author |
DAVID LEHMANN1, SANDRA LABORDE1, SEBASTIEN RIBAULT1, MARTHA ROOK2*
*Corresponding author; 1. Millipore Corporation, European Development & Industrialization
39 route industrielle de la Hardt, Molsheim, F-67120, France
2. Millipore Corporation, 80 Ashby Road, Bedford, MA 01730, USA |
| Citation Information |
CHEMISTRY TODAY, : (2009) |
| Pub Med ID |
3/2009 |
| |
|
Abstract
Pharmaceutical, biotech, and food/beverage indust ries
predominantly use traditional cultured-based assays to detect
microbial contamination in their products. There are significant
benefits to the availability of faster methods that will provide
equivalent or better results. Here, we present an approach to
couple membrane filtration-based concentration and capture
of organisms from complex liquid samples with purification and
detection of the organism’s nucleic acid using Real-Time
Transcription-Mediated Amplification (Real-Time TMA; Gen-
Probe Incorporated). This method can potentially be applied
to detect a wide variety of micro organisms, including
Mycoplasma, bacteria, fungi and viruses. Using a Mycoplasma
assay we were able to detect 5CFU/mL of M. fermentans from
a complex mammalian cell culture sample.
