SNAP i.d. Protein Detection System
The SNAP i.d. system is compatible with your favorite membrane and detection method!
The SNAP i.d. system achieves high quality results without any additional reagent consumption (e.g., antigen, antibody or detection reagents). The system’s unique design enables the use of small volumes for antibody incubations with either polyvinylidene difluoride (PVDF) or nitrocellulose blotting membranes. Because it uses a vacuum to actively drive reagents through the membrane, blocking and washing steps are achieved thoroughly and rapidly. The SNAP i.d. system is compatible with fluorescent, chemiluminescent or chromagenic detection methods. Moreover, the sequence of steps required to process a western blot with the SNAP i.d. system is identical to that used in traditional immunodetection.
Any Membrane
Any Detection Method
Low, Medium or Highly Expressed Proteins
A variety of cell lysates from rat liver, cancer or stem cells, and human serum, were resolved by SDS-PAGE, transferred to Immobilon-P membrane and subjected to immunodetection according to the SNAP i.d. protocol. Proteins of different molecular weight and abundance were visualized after incubating the blots with Immobilon Western HRP substrate and exposing to x-ray film for one to five minutes.
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