Anti-Astrocytomas, a marker for Low Grade Astrocytoma, clone J1-31
Recommended Replacement for: MAB5314| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, H, M, R | WB, IC, IH(P) | Mouse | Ascites | Monoclonal Antibody |
Description:
Anti-Astrocytomas, a marker for Low Grade Astrocytoma, clone J1-31
Clone:
J1-31
Trade Name:
Chemicon (Millipore)
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Western blot: reducing conditions, recognizes a 30kDa protein {Singh, R et al (1986) Biosci Rep 6(1):73-80.
Immunohistochemistry The antibody works on paraffin embedded tissue sections.
Immunocytochemistry
Optimal working dilutions must be determined by the end user.
Immunohistochemistry The antibody works on paraffin embedded tissue sections.
Immunocytochemistry
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Guinea Pig
- Human
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Antibody Sub-Category:
Neuronal & Glial Markers
Specificity:
The antibody recognizes an intracellular protein antigen (MW 30 kDa) expressed by human and rat astrocytes and other specialized glia (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS).The antibody has recently been found to be a specific marker for low grade astrocytoma in human brain tissue. The antibody is able to distinguish between low grade astrocytoma and normal reactive gliosis (patent application filed).
Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.
Monoclonal antibody J1-31 was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at approximately 30,000 daltons under reducing conditions for sodium dodecyl sulfate gel electrophoresis (Singh et al.: Bioscience Reports 6:73-79, 1986). By immunofluorescence microscopy, MAb J1-31 stains those cells that are also stained by antiserum to glial fibrillary acidic protein (GFAP), namely astrocytes, retinal Muller cells, and tanycytes in the ependyma (Predy et al.: Bioscience Reports 7:491-502, 1987). In addition, MAb J1-31 stains ciliated ependymal cells that do not express GFAP {Malhotra, SK (1989) J Neurosci Res. 22(1):36-49}.
Antibody Category:
Neuroscience
Format:
Ascites
Presentation:
Ascites fluid. Liquid.
Isotype:
IgM
Immunogen:
Human cerebral white matter plaque materials from a multiple sclerosis patient.
Antibody Type:
Monoclonal Antibody
Epitope:
a marker for Low Grade Astrocytoma
Replaces:
MAB5314
Storage Conditions:
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Host:
Mouse