Anti-Glial Fibrillary Acidic Protein, clone GA5
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, Ch, H, M, Po, R, Rb | WB, IC, IH, IH(P) | Mouse | Ascites | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander di ... see more »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander di ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein, clone GA5
Clone:
GA5
Trade Name:
Chemicon (Millipore)
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Immunohistochemistry:1:400-1:800 on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum).
Immunocytochemistry on cultured mammalian cells.
Immunoblotting: 51kDa
Optimal working dilutions must be determined by the end user.
Immunocytochemistry on cultured mammalian cells.
Immunoblotting: 51kDa
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Bovine
- Chicken
- Human
- Mouse
- Pig
- Rat
- Rabbit
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
GFAP
Antibody Sub-Category:
Neuronal & Glial Markers
Concentration:
Variable
Specificity:
Recognizes Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Antibody Category:
Neuroscience
Molecular Weight:
~ 51 kDa
Presentation:
Ascites fluid with 15 mM sodium azide as a preservative.
Format Code:
Asc
Control:
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purification Method:
UnPurified
Gene Symbol:
- GFAP
- FLJ45472
Format:
Ascites
Isotype:
IgG1
Immunogen:
Purified GFAP from porcine spinal cord.
Antibody Type:
Monoclonal Antibody
Host:
Mouse