Anti-Glutamine Synthetase, clone GS-6
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R, Sh | ELISA, WB, IH, IH(P) | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P20749 # Could be a transcriptional activating factor. Functions as a form of I-kappa-B specific for NF-kappa-B p50 subunit inhibiting its translocation to the nucleus.
SIZE: 446 amino acids; 46800 Da
SUBUNIT: Interacts with N4BP2 and COPS5.
SUBCELLULAR LOCATION: Nucleus.
PTM: Activated by phosphorylation.
DISEASE:SwissProt: P20749 # A chromosomal aberration involving BCL3 may be a cause of B-cell chronic ly ... see more »
SIZE: 446 amino acids; 46800 Da
SUBUNIT: Interacts with N4BP2 and COPS5.
SUBCELLULAR LOCATION: Nucleus.
PTM: Activated by phosphorylation.
DISEASE:SwissProt: P20749 # A chromosomal aberration involving BCL3 may be a cause of B-cell chronic ly ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene is a proto-oncogene candidate. It is identified by its translocation into the immunoglobulin alpha-locus in some cases of B-cell leukemia. The protein encoded by this gene contains seven ankyrin repeats, which are most closely related to those found in I kappa B proteins. This protein functions as a transcriptional co-activator that activates through its association with NF-kappa B homodimers. The expression of this gene can be induced by NF-kappa B, which forms a part of the autoregul
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Description:
Anti-Glutamine Synthetase, clone GS-6
Clone:
GS-6
Trade Name:
Chemicon (Millipore)
Background Information:
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 45 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. The level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.
Key Applications:
- ELISA
- Western Blotting
- Immunohistochemistry
- Immunohistochemistry (Paraffin)
Application Notes:
Western blot: 1:1,000-1:5,000
Immunohistochemistry on rat brain
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry on rat brain
ELISA
Optimal working dilutions must be determined by end user.
Species Reactivity:
- Human
- Mouse
- Rat
- Sheep
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Antibody Sub-Category:
Neurofilament & Neuron Metabolism
Concentration:
1 mg/mL
Purification Method:
Protein A Purified
Specificity:
Reacts strongly with a single band on Western blots of sheep brain and rat brain. Glutamine synthetase, an enzyme that catalyzes the amination of glutamic acid to form glutamine, is found in mammals as an octamer of eight identical 45 kDa subunits. Glutamine synthetase activity has been shown to be a useful marker of astrocytes and an important differentiation feature in retina. Glutamine synthetase is also considered to be a key enzyme in the recycling of the neurotransmitter glutamate.
Antibody Category:
Neuroscience
Gene Symbol:
- BCL3
- D19S37
- BCL4
Format:
Purified
Molecular Weight:
45 kDa
Presentation:
Purified immunoglobulin. Liquid in buffer containing 50% glycerol, BSA and 0.09% sodium azide.
Isotype:
IgG2a
Immunogen:
Glutamine synthetase purified from sheep brain.
Format Code:
Pur
Antibody Type:
Monoclonal Antibody
Control:
Rat brain tissue, rat brain cytosolic fraction extract
Storage Conditions:
Maintain antibody at 2–8°C in undiluted aliquots for up to 12 months. Avoid repeated freeze-thaw cycles.
Host:
Mouse