Anti-Glial Fibrillary Acidic Protein, clone GF12-24
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| B, H, M, R | WB, IH(P) | Mouse | Purified | Monoclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander di ... see more »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander di ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein, clone GF12-24
Clone:
GF12-24
Trade Name:
Chemicon (Millipore)
Background Information:
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Key Applications:
- Western Blotting
- Immunohistochemistry (Paraffin)
Application Notes:
Classification of brain tumours
Suitable for formalin fixed paraffin wax embedded tissue sections
Pre-natal diagnosis of neural tube defects
Western blotting
Optimal working dilutions must be determined by the end user.
Suitable for formalin fixed paraffin wax embedded tissue sections
Pre-natal diagnosis of neural tube defects
Western blotting
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Bovine
- Human
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
GFAP
Antibody Sub-Category:
Neuronal & Glial Markers
Specificity:
This antibody reacts with a 50 kDa GFAP in human brain sections. No cross-reactivity with other intermediate filaments has been observed. GFAP is a specific marker for astroglial cells.
KNOWN SPECIES CROSS REACTIVITY: Recognizes bovine, rat and mouse
KNOWN SPECIES CROSS REACTIVITY: Recognizes bovine, rat and mouse
Antibody Category:
Neuroscience
Gene Symbol:
- GFAP
- FLJ45472
Format:
Purified
Replaced By:
MAB3402
Presentation:
The monoclonal is presented as 50µg lyophilysed material. Reconstitute with 1ml distilled water. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Isotype:
IgG2a
Antibody Type:
Monoclonal Antibody
Storage Conditions:
For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Host:
Mouse