Anti-Brain lipid-binding protein
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB, IH | Rabbit | Serum | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: O15540 # B-FABP could be involved in the transport of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development. It is required for the establishment of the radial glial fiber system in developing brain, a system that is necessary for the migration of immature neurons to establish cortical layers (By similarity).
SIZE: 132 amino acids; 14889 Da
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: ... see more »
SIZE: 132 amino acids; 14889 Da
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: ... see more »
Entrez Gene Number:
Entrez Gene Summary
The protein encoded by this gene is a brain fatty acid binding protein. Fatty acid binding proteins (FABPs) are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. FABPs are thought to play roles in fatty acid uptake, transport, and metabolism.
Description:
Anti-Brain lipid-binding protein
Trade Name:
Chemicon (Millipore)
Background Information:
BLBP (Brain lipid binding protein) belongs to a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. BLBPs play essential roles in fatty acid uptake, transport and metabolism. BLBP can be used as a molecular marker for radial glia (a major neural progenitor cell type and a scaffolding supporting neuronal migration) in the developing central nervous system. Radial glia play an essential role in the generation of the cerebral cortex through their function as neuronal precursors and as neuronal migration guides.
Key Applications:
- Western Blotting
- Immunohistochemistry
Application Notes:
Immunoblotting: 1:1000-1:5000 using ECL. Reacts with the ~15 kDa BLBP.
Immunohistochemistry: 1:500-1:1500. The antibody works on cryostat/vibratome sectioned tissue. It has been reported that the antibody works on paraffin-embedded materials.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:500-1:1500. The antibody works on cryostat/vibratome sectioned tissue. It has been reported that the antibody works on paraffin-embedded materials.
Optimal working dilutions must be determined by the end user.
Species Reactivity:
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
BLBP, FABP7
Antibody Sub-Category:
Neuronal & Glial Markers
Concentration:
Variable
Purification Method:
UnPurified
Specificity:
Recognizes Brain Lipid-Binding Protein (BLBP). The antibody is specific to radial glia (embryonically) and astrocytes postnatally.
Antibody Category:
Neuroscience
Gene Symbol:
- FABP7
- B-FABP
- MRG
- BLBP
- DKFZp547J2313
- FABPB
Format:
Serum
Molecular Weight:
~15 kDa
Presentation:
Rabbit Serum. Liquid. Contains no preservative.
Immunogen:
Recombinant whole BLBP.
Antibody Type:
Polyclonal Antibody
Control:
- Brain (mouse) tissue lysate - normal tissue, 0 days old. Rat spinal cord from embryonic day 16
- young zebrafish spinal cord tissue
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Rabbit