Anti-Glial Fibrillary Acidic Protein
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R | WB, IC, IH | Chicken | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: SwissProt: P14136 # GFAP, a class-III intermediate filament, is a cell- specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander di ... see more »
SIZE: 432 amino acids; 49880 Da
SUBUNIT: Isoform 3 interacts with N-terminus of PSEN1.
SUBCELLULAR LOCATION: Cytoplasm. Note=Associated with intermediate filaments.
DISEASE: SwissProt: P14136 # Defects in GFAP are a cause of Alexander di ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant has been described, but its full length sequence has not been determined.
Description:
Anti-Glial Fibrillary Acidic Protein
Trade Name:
Chemicon (Millipore)
Key Applications:
- Western Blotting
- Immunocytochemistry
- Immunohistochemistry
Application Notes:
Western blot: 1:100-1:500 using ECL. Recognizes a band of 55 kDa. May also react with a band of 50 kDa.
Immunohistochemistry: 1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:200-1:1000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
Immunohistochemistry: 1:100-1:500 on frozen tissue sections. Suggested fixative is 4% paraformaldehyde.
Immunocytochemistry: 1:200-1:1000 on cells in tissue culture fixed for one minute in 3.7% formalin and one minute in -20°C methanol.
Optimal working dilutions must be determined by the end user.
STAINING PATTERN:
The antibody stains sharply defined cytoplasmic filaments of astroglia cells in tissue culture. Stains processes of astrocytes in sections of brain tissues.
Species Reactivity:
- Mouse
- Rat
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
GFAP
Antibody Sub-Category:
Neuronal & Glial Markers
Concentration:
Variable
Purification Method:
Immunoaffinity Purified
Specificity:
Recognizes Glial fibrillary acidic protein (GFAP). Reacts with both native and recombinant protein.
Antibody Category:
Neuroscience
Gene Symbol:
- GFAP
- FLJ45472
Format:
Affinity Purified
Molecular Weight:
55 kDa
Presentation:
Purified chicken IgY. Liquid in PBS containing 5 mM sodium azide.
Immunogen:
Purified bovine GFAP.
Format Code:
APur
Antibody Type:
Polyclonal Antibody
Control:
Astrocytes, astrocytomas, neural stem cells.
Storage Conditions:
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Chicken