Anti-MBD1 (methyl-CpG binding domain protein 1)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB | Rabbit | Affinity Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
Transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binding is abolished by the presence of 7-mG that is produced by DNA damage by methylmethanesulfonate (MMS). Acts as transcriptional repressor and plays a role in gene silencing by recruiting AFT7IP, which in turn recruits factors such as the histone methyltransferase SETDB1. Probably forms a complex with SETDB1 and ATF7IP that represses transcription and
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Entrez Gene Number:
Entrez Gene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD
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Description:
Anti-MBD1 (methyl-CpG binding domain protein 1)
Trade Name:
Upstate (Millipore)
Background Information:
MBD1 (methyl-CpG binding domain protein 1) is a transcriptional repressor that binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD1 is part of the MeCP1 complex where it acts as a transcriptional repressor and plays a role in gene silencing. MBD1 forms a complex with the histone methyltransferase SETDB1 and ATF7IP that represses transcription and couples DNA methylation and histone Lys-9 trimethylation. During DNA replication, it recruits SETDB1 to form a S phase-specific complex that facilitates methylation of Lys9 on histone H3 during replication-coupled chromatin assembly. MBD1 is associated with euchromatic regions of the chromosomes and with telomeric regions
Key Applications:
Western Blotting
Species Reactivity:
Human
Species Reactivity Note:
Human
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
Methyl-CpG-binding domain protein 1
PCM1
RFT
CXXC3
PCM1
RFT
CXXC3
Antibody Sub-Category:
- Chromatin Biology
- Transcription Factors
Concentration:
1 mg/mL
Purification Method:
immunoaffinity purified
Specificity:
Recognizes MBD1
Antibody Category:
Nuclear Function
Gene Symbol:
- CXXC3
- CXXC-type zinc finger protein 3
- Methyl-CpG-binding protein MBD1
- Regulator of fibroblast growth factor 2 (FGF-2) transcription 2
- PCM1
- MBD1
Format:
Affinity Purified
Molecular Weight:
60 kda
Presentation:
Immunoaffinity purified rabbit IgG in PBS containing 0.1% sodium azide, 30% glycerol
Isotype:
IgG
Immunogen:
KLH conjugated synthetic peptide (RKRQVGPQSGEVRKEAPR(C) corresponding to a.a. 96-113 of human MBD1.
Quality Assurance:
Routinely evaluated by immunoblot.
Antibody Type:
Polyclonal Antibody
Epitope:
a.a. 96-113
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance
Host:
Rabbit