Anti-TFIIB, (N-term)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rabbit | Purified | Polyclonal Antibody |
UniProt Number:
UniProt Summary
FUNCTION: General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.Cofactor Binds 1 zinc ion per subunit.
SUBUNITS: Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 By similarity.
SUBCELLULAR LOCATION: Nucleus.
SEQUENCE SIMILARITIES: Belongs to the TFIIB fa ... see more »
SUBUNITS: Associates with TFIID-IIA (DA complex) to form TFIID-IIA-IIB (DAB-complex) which is then recognized by polymerase II. Interacts with the transcription elongation factor TCEA2. Interacts with HIV-1 Vpr. Interacts with GPBP1 By similarity.
SUBCELLULAR LOCATION: Nucleus.
SEQUENCE SIMILARITIES: Belongs to the TFIIB fa ... see more »
Entrez Gene Number:
Entrez Gene Summary
This gene encodes the general transcription factor IIB,one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.
Description:
Anti-TFIIB (N-Term)
Trade Name:
Upstate (Millipore)
Background Information:
TFIIB (GTF2B, TF2B, general transcription factor IIB) is a ubiquitously expressed transcription factor. TFIIB is essential for the formation of the RNA Polymerase II initiation complex. It is localized to the nucleus where it forms a complex with both TFIIA and TFIID (DAB complex). There it serves a linker between TFIID and RNA Polymerase II. TFIIB is also known to interact with POU3F2.
Key Applications:
Western Blotting
Species Reactivity:
- Human
- Mouse
- Rat
Species Reactivity Note:
Human. Mouse and Rat are expected to react as they share 99% homology.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alternate Names:
- Transcription initiation factor IIB
- General transcription factor IIB
- RNA polymerase II transcription factor IIB
Concentration:
1.0 mg/mL
Specificity:
Detects TFIIB.
Purification Method:
Antigen Affinity Purified
Antibody Category:
- Cell Signaling
- Nuclear Function
Gene Symbol:
- GTF2B
- TF2B
- TFIIB
Format:
Purified
Molecular Weight:
GST-tagged recombinant protein including amino acids 2-159 of TFIIB
Presentation:
Affinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4) 15mM NaCl, and 0.05% NaN3.
Immunogen:
GST-tagged recombinant protein including amino acids 2-159 of TFIIB
Quality Assurance:
Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected TFIIB at 1:500 dilution in HeLa nulear extract lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Western Blot Analysis:
This lot detected TFIIB at 1:500 dilution in HeLa nulear extract lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Antibody Type:
Polyclonal Antibody
Epitope:
N-Term
Control:
HeLa nuclear extract
Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Host:
Rabbit